LOCUS       HAS2-pGEc2              6882 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(6360..6489)
                     /note="3'-ITR"
     misc_feature    complement(6058..6272)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    5510..6051
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    4689..4711
                     /note="attB2"
     misc_feature    5489..5494
                     /note="BstB1 site"
     misc_feature    4712..5407
                     /note="Super GFP"
     misc_feature    5408..5458
                     /note="AviTag"
     misc_feature    5459..5482
                     /note="His Tag"
     misc_feature    3011..4666
                     /note="OPTIMIZED HAS2  coding region"
     PCR_frame       3011..4688
                     /note="Reading Frame"
                     /set="Set 1"
     misc_feature    4667..4688
                     /note="TEV"
     vector          join(4697..6882,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..4696
                     /source="HAS2 pDONR"
                     /type="Entry Clone"
     source          1..6882
                     /dnas_title="HAS2-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGCACTGCG AGAGATTTCT GTGCATCCTG CGGATCATCG GCACCACCCT
     3061 GTTTGGAGTG TCTCTGCTGC TGGGCATCAC AGCCGCCTAT ATCGTGGGCT ACCAGTTCAT
     3121 CCAGACCGAC AACTACTACT TCAGCTTCGG CCTGTACGGC GCCTTTCTGG CCTCTCACCT
     3181 GATCATCCAG TCCCTGTTCG CCTTCCTGGA ACACCGGAAG ATGAAGAAGT CCCTGGAAAC
     3241 CCCCATCAAG CTGAACAAGA CCGTGGCCCT GTGTATCGCC GCCTACCAGG AAGATCCCGA
     3301 CTACCTGAGA AAGTGCCTGC AGAGCGTGAA GAGACTGACC TACCCTGGCA TCAAGGTGGT
     3361 CATGGTCATC GACGGCAACA GCGAGGACGA CCTGTACATG ATGGACATCT TCAGCGAAGT
     3421 GATGGGCCGG GACAAGAGCG CCACCTACAT CTGGAAGAAC AACTTCCACG AGAAGGGCCC
     3481 TGGCGAGACA GACGAGAGCC ACAAAGAAAG CTCTCAGCAC GTGACCCAGC TGGTGCTGAG
     3541 CAACAAGAGC ATCTGTATCA TGCAGAAGTG GGGCGGCAAG CGGGAAGTCA TGTATACCGC
     3601 TTTTAGAGCC CTGGGCAGAA GCGTGGACTA CGTCCAGGTC TGCGATAGCG ACACAATGCT
     3661 GGATCCTGCC AGCAGCGTGG AAATGGTCAA GGTGCTGGAA GAGGACCCTA TGGTCGGAGG
     3721 TGTCGGCGGA GATGTGCAGA TCCTGAACAA ATACGACAGC TGGATCAGCT TCCTGTCCAG
     3781 CGTGCGGTAC TGGATGGCCT TCAATATCGA GAGAGCCTGC CAGAGCTACT TCGGCTGCGT
     3841 GCAGTGTATC TCTGGCCCCC TGGGAATGTA CAGAAACAGC CTGCTGCACG AGTTCGTCGA
     3901 GGACTGGTAC AACCAGGAAT TCATGGGCAA CCAGTGCAGC TTTGGCGACG ACAGGCACCT
     3961 GACCAATAGA GTGCTGTCTC TGGGCTACGC CACCAAGTAC ACCGCCAGAT CCAAGTGTCT
     4021 GACCGAGACA CCTATCGAGT ACCTGCGGTG GCTGAATCAG CAGACCAGAT GGTCCAAGTC
     4081 CTACTTTAGA GAGTGGCTGT ACAACGCCAT GTGGTTCCAC AAGCACCACC TGTGGATGAC
     4141 CTACGAGGCT ATTATCACCG GCTTTTTCCC ATTCTTCCTG ATCGCCACCG TCATTCAGCT
     4201 GTTCTACCGG GGCAAGATCT GGAATATCCT GCTGTTCCTG CTGACCGTGC AGCTCGTGGG
     4261 ACTGATCAAG TCCAGCTTTG CCAGCTGCCT GCGGGGCAAC ATCGTGATGG TGTTCATGAG
     4321 CCTGTACTCC GTGCTGTATA TGTCCTCCCT GCTGCCCGCC AAGATGTTCG CCATTGCCAC
     4381 AATCAACAAG GCCGGCTGGG GCACCAGCGG CAGAAAGACA ATCGTGGTCA ACTTCATCGG
     4441 CCTGATCCCC GTGTCCGTGT GGTTCACTAT TCTGCTGGGA GGCGTGATCT TCACCATCTA
     4501 CAAAGAGAGC AAGCGGCCTT TCAGCGAGTC CAAGCAGACA GTGCTGATCG TGGGAACCCT
     4561 GCTGTACGCC TGTTACTGGG TCATGCTGCT CACCCTGTAC GTGGTCCTGA TTAACAAGTG
     4621 CGGCAGACGG AAGAAGGGAC AGCAGTACGA CATGGTGCTC GACGTCgaaa acttgtactt
     4681 tcaaggctCC AGCTTTCTTG TACAAAGTGG TATGAGCAAG GGCGAGGAAC TGTTCACCGG
     4741 CGTGGTGCCC ATCCTGGTGG AACTGGATGG CGACGTGAAC GGCCACAAGT TCTCTGTGCG
     4801 GGGAGAGGGC GAAGGCGACG CCACAAATGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC
     4861 CGGCAAGCTG CCCGTGCCTT GGCCTACCCT CGTGACCACA CTGACCTACG GCGTGCAGTG
     4921 CTTCAGCAGA TACCCCGACC ATATGAAGCG GCACGACTTC TTCAAGAGCG CCATGCCCGA
     4981 GGGCTACGTG CAGGAACGGA CCATCAGCTT CAAGGACGAC GGCACCTACA AGACCAGAGC
     5041 CGAAGTGAAG TTCGAGGGCG ACACCCTCGT GAACCGGATC GAGCTGAAGG GCATCGATTT
     5101 CAAAGAGGAC GGCAACATCC TGGGCCACAA GCTGGAGTAC AACTTCAACA GCCACAACGT
     5161 GTACATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC AACTTCAAGA TCCGGCACAA
     5221 CGTGGAAGAT GGCAGCGTGC AGCTGGCCGA CCACTACCAG CAGAACACCC CCATCGGAGA
     5281 TGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGAGCGTGC TGAGCAAGGA
     5341 CCCCAACGAG AAGCGGGACC ACATGGTGCT GCTGGAATTC GTGACCGCCG CTGGCATCAC
     5401 CCACGGCATG AGCGGCCTGA ACGACATCTT CGAGGCCCAG AAAATCGAGT GGCACGAGCA
     5461 CCACCATCAT CACCATCACC ACTGATGATT CGAAAAATAA TAAGGATCCA ATCAACCTCT
     5521 GGATTACAAA ATTTGTGAAA GATTGACTGG TATTCTTAAC TATGTTGCTC CTTTTACGCT
     5581 ATGTGGATAC GCTGCTTTAA TGCCTTTGTA TCATGCTATT GCTTCCCGTA TGGCTTTCAT
     5641 TTTCTCCTCC TTGTATAAAT CCTGGTTGCT GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT
     5701 CAGGCAACGT GGCGTGGTGT GCACTGTGTT TGCTGACGCA ACCCCCACTG GTTGGGGCAT
     5761 TGCCACCACC TGTCAGCTCC TTTCCGGGAC TTTCGCTTTC CCCCTCCCTA TTGCCACGGC
     5821 GGAACTCATC GCCGCCTGCC TTGCCCGCTG CTGGACAGGG GCTCGGCTGT TGGGCACTGA
     5881 CAATTCCGTG GTGTTGTCGG GGAAGCTGAC GTCCTTTCCA TGGCTGCTCG CCTGTGTTGC
     5941 CACCTGGATT CTGCGCGGGA CGTCCTTCTG CTACGTCCCT TCGGCCCTCA ATCCAGCGGA
     6001 CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT GCGGCCTCTT CCGCGTCTTC GAGATCTGCC
     6061 TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG
     6121 ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT
     6181 TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG
     6241 GATTGGGAAG ACAATAGCAG GCATGCTGGG GACTCGAGTT AAGGGCGAAT TGCCGATTAG
     6301 GATCTTCCTA GAGCATGGCT ACGTAGATAA GTAGCATGGC GGGTTAATCA TTAACTACAA
     6361 GGAACCCCTA GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC
     6421 CGGGCGACCA AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG
     6481 AGCGCGCAGC CTTAATTAAG AGCTCATGGC GCGCCTAGGC CTTGACGGCC TTCCGCCAAT
     6541 TCGCCCTATA GTGAGTCGTA TTACGTCGCG CTCACTGGCC GTCGTTTTAC AACGTCGTGA
     6601 CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG
     6661 CTGGCGTAAT AGCGAAGAGG CCCGCACCGA AACGCCCTTC CCAACAGTTG CGCAGCCTGA
     6721 ATGGCGAATG GGAGCGCCCT GTAGCGGCCA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT
     6781 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA
     6841 TTTAACGCGA ATTTTAACAA AATATTAACG CTTACAATTT AG
//

